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recombinant carrier free ha tagged mouse cd40l  (R&D Systems)


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    Structured Review

    R&D Systems recombinant carrier free ha tagged mouse cd40l
    Recombinant Carrier Free Ha Tagged Mouse Cd40l, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+carrier+free+ha+tagged+mouse+cd40l/Recombinant+Mouse+CD40+Ligand%2FTNFSF5+(HA-tag)+Protein%2C+CF/us12589145-924-19-25
    Average 94 stars, based on 7 article reviews
    recombinant carrier free ha tagged mouse cd40l - by Bioz Stars, 2026-10
    94/100 stars

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    Isolation:

    Article Title: Systems to produce B cells genetically modified to express selected antibodies
    Article Snippet: Base B cell medium included RPMI medium with 10% Fetal calf serum (Hyclone), 10 mM HEPES (Gibco), 1 mM sodium pyruvate, (Gibco), 55 μM p-mercaptoethanol (Sigma), and 100 U/ml penicillin plus 100 μg/mL streptomycin (Gibco) except in antibiotic free steps as noted. .. B cells were isolated from spleen and lymph nodes via negative selection with magnetic beads (Miltenyi) and cultured for 24 hours at 2×106/ml in B cell medium supplemented with 100 ng/ml recombinant carrier free HA-tagged mouse CD40L (R&D systems), 100 ng/ml anti-HA antibody (clone 543851, R&D systems), and 4 ng/ml mouse IL-4 (R&D systems). ..

    Selection:

    Article Title: Systems to produce B cells genetically modified to express selected antibodies
    Article Snippet: Base B cell medium included RPMI medium with 10% Fetal calf serum (Hyclone), 10 mM HEPES (Gibco), 1 mM sodium pyruvate, (Gibco), 55 μM p-mercaptoethanol (Sigma), and 100 U/ml penicillin plus 100 μg/mL streptomycin (Gibco) except in antibiotic free steps as noted. .. B cells were isolated from spleen and lymph nodes via negative selection with magnetic beads (Miltenyi) and cultured for 24 hours at 2×106/ml in B cell medium supplemented with 100 ng/ml recombinant carrier free HA-tagged mouse CD40L (R&D systems), 100 ng/ml anti-HA antibody (clone 543851, R&D systems), and 4 ng/ml mouse IL-4 (R&D systems). ..

    Magnetic Beads:

    Article Title: Systems to produce B cells genetically modified to express selected antibodies
    Article Snippet: Base B cell medium included RPMI medium with 10% Fetal calf serum (Hyclone), 10 mM HEPES (Gibco), 1 mM sodium pyruvate, (Gibco), 55 μM p-mercaptoethanol (Sigma), and 100 U/ml penicillin plus 100 μg/mL streptomycin (Gibco) except in antibiotic free steps as noted. .. B cells were isolated from spleen and lymph nodes via negative selection with magnetic beads (Miltenyi) and cultured for 24 hours at 2×106/ml in B cell medium supplemented with 100 ng/ml recombinant carrier free HA-tagged mouse CD40L (R&D systems), 100 ng/ml anti-HA antibody (clone 543851, R&D systems), and 4 ng/ml mouse IL-4 (R&D systems). ..

    Cell Culture:

    Article Title: Systems to produce B cells genetically modified to express selected antibodies
    Article Snippet: Base B cell medium included RPMI medium with 10% Fetal calf serum (Hyclone), 10 mM HEPES (Gibco), 1 mM sodium pyruvate, (Gibco), 55 μM p-mercaptoethanol (Sigma), and 100 U/ml penicillin plus 100 μg/mL streptomycin (Gibco) except in antibiotic free steps as noted. .. B cells were isolated from spleen and lymph nodes via negative selection with magnetic beads (Miltenyi) and cultured for 24 hours at 2×106/ml in B cell medium supplemented with 100 ng/ml recombinant carrier free HA-tagged mouse CD40L (R&D systems), 100 ng/ml anti-HA antibody (clone 543851, R&D systems), and 4 ng/ml mouse IL-4 (R&D systems). ..

    Recombinant:

    Article Title: Systems to produce B cells genetically modified to express selected antibodies
    Article Snippet: Base B cell medium included RPMI medium with 10% Fetal calf serum (Hyclone), 10 mM HEPES (Gibco), 1 mM sodium pyruvate, (Gibco), 55 μM p-mercaptoethanol (Sigma), and 100 U/ml penicillin plus 100 μg/mL streptomycin (Gibco) except in antibiotic free steps as noted. .. B cells were isolated from spleen and lymph nodes via negative selection with magnetic beads (Miltenyi) and cultured for 24 hours at 2×106/ml in B cell medium supplemented with 100 ng/ml recombinant carrier free HA-tagged mouse CD40L (R&D systems), 100 ng/ml anti-HA antibody (clone 543851, R&D systems), and 4 ng/ml mouse IL-4 (R&D systems). ..

    Article Title: B cells engineered to express pathogen-specific antibodies protect against infection
    Article Snippet: .. After AAV infection, B cells were expanded for an additional 48 hours with B cell medium supplemented with 100 ng/mL recombinant carrier free HA-tagged mouse CD40L, 100 ng/mL αHA antibody, 4 ng/mL mouse IL-4 (R&D systems), and 20 ng/mL mouse IL-21 (BioLegend). ..

    Article Title: Systems to produce B cells genetically modified to express selected antibodies
    Article Snippet: .. After electroporation, B cells were expanded for an additional 48 hours with B cell medium supplemented with 100 ng/ml recombinant carrier free HA-tagged mouse CD40L (R&D systems), 100 ng/ml anti-HA antibody (clone 543851, R&D systems), 4 ng/ml mouse IL-4 (R&D systems), and 20 ng/ml mouse IL-21. (Biolegend). .. For secondary expansion, B cells were co-cultured with irradiated (80 gy) NIH 3T3-CD40L feeder cells in the presence of 20 ng/ml mouse IL-21. (Biolegend).

    Bioprocessing:

    Article Title: B cells engineered to express pathogen-specific antibodies protect against infection
    Article Snippet: .. After AAV infection, B cells were expanded for an additional 48 hours with B cell medium supplemented with 100 ng/mL recombinant carrier free HA-tagged mouse CD40L, 100 ng/mL αHA antibody, 4 ng/mL mouse IL-4 (R&D systems), and 20 ng/mL mouse IL-21 (BioLegend). ..

    Infection:

    Article Title: B cells engineered to express pathogen-specific antibodies protect against infection
    Article Snippet: .. After AAV infection, B cells were expanded for an additional 48 hours with B cell medium supplemented with 100 ng/mL recombinant carrier free HA-tagged mouse CD40L, 100 ng/mL αHA antibody, 4 ng/mL mouse IL-4 (R&D systems), and 20 ng/mL mouse IL-21 (BioLegend). ..

    Electroporation:

    Article Title: Systems to produce B cells genetically modified to express selected antibodies
    Article Snippet: .. After electroporation, B cells were expanded for an additional 48 hours with B cell medium supplemented with 100 ng/ml recombinant carrier free HA-tagged mouse CD40L (R&D systems), 100 ng/ml anti-HA antibody (clone 543851, R&D systems), 4 ng/ml mouse IL-4 (R&D systems), and 20 ng/ml mouse IL-21. (Biolegend). .. For secondary expansion, B cells were co-cultured with irradiated (80 gy) NIH 3T3-CD40L feeder cells in the presence of 20 ng/ml mouse IL-21. (Biolegend).



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    94
    R&D Systems recombinant carrier free ha tagged mouse cd40l
    Recombinant Carrier Free Ha Tagged Mouse Cd40l, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+carrier+free+ha+tagged+mouse+cd40l/Recombinant+Mouse+CD40+Ligand%2FTNFSF5+(HA-tag)+Protein%2C+CF/us12589145-924-19-25
    Average 94 stars, based on 1 article reviews
    recombinant carrier free ha tagged mouse cd40l - by Bioz Stars, 2026-10
    94/100 stars
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